Unusual Features of Sodium Taurocholate Cotransporting Polypeptide as a Hepatitis B Virus Receptor

• DOI: 10.1128/jvi.01153-16
• Publication Date: 2016-07-07T01:38:44Z
• Authors: Jisu Li; Li Zong; Camille Sureau; Luke Barker; Jack R. Wands; Shuping Tong
• Keywords: HBeAg; Hepatitis B

Cell culture (cc)-derived hepatitis B virus (HBV) can infect differentiated HepaRG cells, but efficient infection requires addition of polyethylene glycol (PEG) during inoculation. Identification sodium taurocholate cotransporting polypeptide (NTCP) as an HBV receptor enabled ccHBV NTCP reconstituted HepG2 although very little surface antigen (HBsAg) is produced. We found by patient serum-derived (sHBV), which required purification viral particles through ultracentrifugation or PEG precipitation, was independent and much more in cells than HepG2/NTCP cells. In contrast to e (HBeAg), HBsAg not a reliable marker productive sHBV at early time points. A low HBsAg/HBeAg ratio ccHBV-infected attributable dimethyl sulfoxide (DMSO) medium, overexpression, genotype D. released antigens after genome delivery adeno-associated virus, stable expression producing cell line increased mRNAs, proteins, replicative DNA, covalently closed circular DNA. protein despite being driven the cytomegalovirus promoter, markedly DMSO treatment. This least partly explains ability promote such lines. conclusion, appeared inefficient mediate HBV. It could RNA transcription while inhibiting secretion. Efficient PEG-independent permits comparative studies diverse clinical isolates will help identify additional factors on virion promoting attachment hepatocytes.Currently vitro with depends culture-derived inoculated presence glycol. efficiently glycol, represents physiological system. Serum-derived has poor infectivity (NTCP), currently accepted receptor. Moreover, secreted HBV, attributed D added medium. transcription, expression, DNA replication stably transfected increase level transcriptional control promoter. Therefore, this study revealed several unusual features established conditions for serum vitro.