High-Throughput Screening Identifies Inhibitors for Parvovirus B19 Infection of Human Erythroid Progenitors

• DOI: 10.1128/jvi.01326-21
• Publication Date: 2021-10-20T17:41:37Z
• Authors: Kang Ning; Anuradha Roy; Fang Cheng; Peng Xu; Steve Kleiboeker; Carlos R. Escalante; Jingxin Wang; Jianming Qiu
• Keywords: DNA Replication; Erythroid Precursor Cells; 0301 basic medicine; Cell Survival; Replication Origin; Viral Nonstructural Proteins; Virus Replication; Antiviral Agents; Recombinant Proteins; High-Throughput Screening Assays; 3. Good health; Small Molecule Libraries; 03 medical and health sciences; DNA, Viral; Parvovirus B19, Human; Humans; Fluorescent Dyes

B19V encodes a large nonstructural protein, NS1. Its N-terminal domain (NS1N) consisting of amino acids 1 to 176 binds to viral DNA and serves as an endonuclease to nick the viral DNA replication origins, which is a pivotal step in rolling-hairpin-dependent B19V DNA replication.