Ebolavirus VP24 Binding to Karyopherins Is Required for Inhibition of Interferon Signaling

• DOI: 10.1128/jvi.01372-09
• Publication Date: 2009-11-05T02:16:36Z
• Authors: Mathieu Mateo; St. Patrick Reid; Lawrence W. Leung; Christopher F. Ba...; Viktor E. Volchkov
• Keywords: Karyopherin; Ebolavirus; STAT1

The Ebolavirus VP24 protein counteracts alpha/beta interferon (IFN-alpha/beta) and IFN-gamma signaling by blocking the nuclear accumulation of tyrosine-phosphorylated STAT1 (PY-STAT1). According to proposed model, binding members NPI-1 subfamily karyopherin alpha (KPNalpha) localization signal receptors prevents their PY-STAT1, thereby preventing PY-STAT1 accumulation. This study now identifies two domains required for inhibition IFN-beta-induced gene expression We demonstrate that loss function correlates with KPNalpha proteins. Thus, IFN antagonist requires ability interact KPNalpha.