Kaposi's Sarcoma-Associated Herpesvirus Oncoprotein K13 Protects against B Cell Receptor-Induced Growth Arrest and Apoptosis through NF-κB Activation

• DOI: 10.1128/jvi.01393-12
• Publication Date: 2012-12-13T04:28:44Z
• Authors: Ciaren Graham; Hittu Matta; Yanqiang Yang; Han Yi; Yulan Suo; Bhairavi Tolani; Preet M. Chaudhary
• Keywords: Primary effusion lymphoma; RELB; Caspase 8

ABSTRACT Kaposi's sarcoma-associated herpesvirus (KSHV) has been linked to the development of sarcoma, primary effusion lymphoma, and multicentric Castleman's disease (MCD). We have characterized role KSHV-encoded viral FLICE inhibitory protein (vFLIP) K13 in modulation anti-IgM-induced growth arrest apoptosis B cells. demonstrate that protects WEHI 231, an immature B-cell line, against apoptosis. The protective effect was associated with activation NF-κB pathway deficient a mutant three alanine substitutions at positions 58 60 (K13-58AAA) structural homolog, vFLIP E8, both which lack activity. upregulated expression subunit RelB blocked decline c-Myc rise p27 Kip1 also Mcl-1, antiapoptotic member Bcl2 family. Finally, protected mature line Ramos through activation. Inhibition by may contribute KSHV-associated lymphoproliferative disorders.