Template Recognition Mechanisms by Replicase Proteins Differ between Bipartite Positive-Strand Genomic RNAs of a Plant Virus

• DOI: 10.1128/jvi.01754-10
• Publication Date: 2010-10-28T03:20:07Z
• Authors: Hiro-oki Iwakawa; Akira Mine; Kiwamu Hyodo; Mengnan An; Masanori Kaido; Kazuyuki Mise; Tetsuro Okuno
• Keywords: RNA virus

ABSTRACT Recognition of RNA templates by viral replicase proteins is one the key steps in replication process all viruses. However, mechanisms underlying this phenomenon, including primary elements that are recognized proteins, not well understood. Here, we used aptamer pulldown assays with membrane fractionation and protein-RNA coimmunoprecipitation a cell-free translation/replication system to investigate how recognize bipartite genomic RNAs Red clover necrotic mosaic virus (RCNMV). RCNMV bound specifically Y-shaped element (YRE) located 3′ untranslated region (UTR) RNA2, which also interacted 480-kDa complexes contain host proteins. The replicase-YRE interaction recruited RNA2 fraction. Conversely, RNA1 fragments failed interact supplied trans . results suggest interacts coupled their translation. Thus, initial template recognition employed differ between determinants differential mechanism.