Through Its Nonstructural Protein NS1, Parvovirus H-1 Induces Apoptosis via Accumulation of Reactive Oxygen Species

• DOI: 10.1128/jvi.01797-09
• Publication Date: 2010-04-08T01:10:20Z
• Authors: Georgi Hristov; Melanie Krämer; Junwei Li; Nazim El-Andaloussi; Rodrigo Mora; Laurent Daeffler; Hanswalter Zentgraf; Jean Rommelaere; Antonio Marchini
• Keywords: H-1 parvovirus; Parvoviridae Infections; 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; Cell Cycle; Humans; Apoptosis; Viral Nonstructural Proteins; Reactive Oxygen Species; Cell Line; DNA Damage; 3. Good health

ABSTRACT The rat parvovirus H-1 (H-1PV) attracts high attention as an anticancer agent, because it is not pathogenic for humans and has oncotropic oncosuppressive properties. viral nonstructural NS1 protein thought to mediate H-1PV cytotoxicity, but its exact contribution this process remains undefined. In study, we analyzed the effects of on human cell proliferation viability. We show that expression sufficient induce accumulation cells in G 2 phase, apoptosis via caspase 9 3 activation, lysis. Similarly, infected with wild-type arrest phase undergo apoptosis. Furthermore, also both infection lead higher levels intracellular reactive oxygen species (ROS), associated DNA double-strand breaks. Antioxidant treatment reduces ROS strongly decreases NS1- virus-induced damage, cycle arrest, apoptosis, indicating NS1-induced are important mediators cytotoxicity.