Enhanced antigen-specific CD8 T cells contribute to early protection against FMDV through swine DC vaccination

• DOI: 10.1128/jvi.02002-23
• Publication Date: 2024-01-30T14:02:55Z
• Authors: Suyu Mu; Lingbo Chen; Hu Dong; Shuai Li; Yun Zhang; Shuanghui Yin; Yunfei Tian; Yaozhong Ding; Shiqi Sun; Shaobin Shang; Huichen Guo
• Keywords: Cellular immunity

ABSTRACT Foot-and-mouth disease virus (FMDV) remains a challenge for cloven-hooved animals. The currently licensed FMDV vaccines induce neutralizing antibody (NAb)-mediated protection but show defects in the early protection. Dendritic cell (DC) have shown great potency inducing rapid T-cell immunity humans and mice. Whether DC vaccination could enhance against has not been elaborately explored domestic pigs. In this study, we employed as an experimental approach to study roles of cellular Autologous DCs were differentiated from periphery blood mononuclear cells each pig, pulsed with inactivated (iFMDV-DC) treated LPS, then injected into original immune responses protective efficacy elicited by iFMDV-DC examined multicolor flow cytometry tested challenge. results showed that autologous immunization induced predominantly FMDV-specific IFN-γ-producing CD4 + T cytotoxic CD8 (CTLs), high NAb titers, compared vaccine, accelerated development memory cells, which was concomitantly associated virulent strain Such proliferation secondary response after significantly contributed effector cells. These demonstrated induction through is important improving FMDV. Enhancing may facilitate more effective vaccines. IMPORTANCE Although provide NAb-mediated protection, they especially swine augmented This Importantly, conferred rather than NAbs. Our findings highlighted importance enhancing addition Th1 identifying strategy or adjuvant comparable vaccine might be future direction current