The Macrophage Galactose-Type Lectin Can Function as an Attachment and Entry Receptor for Influenza Virus

• DOI: 10.1128/jvi.02014-13
• Publication Date: 2013-11-21T03:15:58Z
• Authors: Wy Ching Ng; Stella Liong; Michelle D. Tate; Tatsuro Irimura; Kaori Denda-Nagai; Andrew G. Brooks; Sarah L. Londrigan; Patrick C. Reading
• Keywords: C-type lectin

ABSTRACT Specific protein receptors that mediate internalization and entry of influenza A virus (IAV) have not been identified for any cell type. Sialic acid (SIA), the primary attachment factor IAV hemagglutinin, is expressed by numerous surface glycoproteins glycolipids, confounding efforts to identify specific involved in infection. Lec1 Chinese hamster ovary (CHO) epithelial cells express SIA bind yet are largely resistant Here, we demonstrate expression murine macrophage galactose-type lectin 1 (MGL1) enhanced Ca 2+ -dependent binding restored permissivity expressing MGL1 were infected presence or absence SIA, indicating can act as a receptor coreceptor with SIA. endocytosis-deficient mediated but less sensitive infection, direct via result cellular Together, these studies glycoprotein an authentic both infectious IAV.