Densovirus Infectious Pathway Requires Clathrin-Mediated Endocytosis Followed by Trafficking to the Nucleus

• DOI: 10.1128/jvi.02401-08
• Publication Date: 2009-02-19T01:54:18Z
• Authors: Agnès Vendeville; Marc Ravallec; Françoise-Xaviè...; Micheline Devise; Doriane Mutuel; Miguel López-Ferber; Philippe Fournier; Thierry Dupressoir; Mylène Ogliastro
• Keywords: Internalization

ABSTRACT Junonia coenia densovirus (JcDNV) is an ambisense insect parvovirus highly pathogenic for lepidopteran pests at larval stages. The potential use of DNVs as biological control agents prompted us to reinvestigate the host range and cellular mechanisms infection. In order understand early events infection, we set up a functional infection assay in cell line pest Lymantria dispar determine intracellular pathway undertaken by JcDNV infect permissive line. Our results show that particles are rapidly internalized into clathrin-coated vesicles slowly traffic within late endocytic compartments. Blocking late-endocytic trafficking or neutralizing pH with drugs inhibited During internalization, disruption cytoskeleton, inhibition phosphatidylinositol 3-kinase blocked movement containing virus nucleus impaired summary, our define first time steps required productive DNV