Viral MicroRNAs Targeting Virus Genes Promote Virus Infection in Shrimp In Vivo

• DOI: 10.1128/jvi.02455-13
• Publication Date: 2013-11-07T02:53:07Z
• Authors: Yaodong He; Kai Yang; Xiaobo Zhang
• Keywords: White spot syndrome

ABSTRACT Viral microRNAs (miRNAs), most of which are characterized in cell lines, have been found to play important roles the virus life cycle avoid attack by host immune system or keep latency state. miRNAs targeting genes can inhibit infection. In this study, vivo findings Marsupenaeus japonicus shrimp revealed that viral could target and further promote The results showed white spot syndrome (WSSV)-encoded WSSV-miR-66 WSSV-miR-68 were transcribed at early stage WSSV When expression was silenced with sequence-specific anti-miRNA oligonucleotides (AMOs), number copies WSSV-infected mortality significantly decreased, indicating two had a great effect on It wsv094 wsv177 targets wsv248 wsv309 WSSV-miR-68. data demonstrate four negative led promotion Therefore, our show novel aspect virus-host interactions.