Scavenger Receptor Class B Is Required for Hepatitis C Virus Uptake and Cross-Presentation by Human Dendritic Cells

• DOI: 10.1128/jvi.02478-07
• Publication Date: 2008-01-24T01:34:06Z
• Authors: Heidi Barth; Eva K. Schnober; Christoph Neumann...; Christine Thumann; Mirjam B. Zeisel; Helmut M. Diepolder; Zongyi Hu; T. Jake Liang; Hubert E. Blum; Robert Thimme; Mélanie Lambotin; Thomas F. Baumert
• Keywords: Scavenger Receptor; Cross-Presentation

Class B scavenger receptors (SR-Bs) bind lipoproteins and play an important role in lipid metabolism. Most recently, SR-B type I (SR-BI) its splicing variant SR-BII have been found to mediate bacterial adhesion cytosolic invasion mammalian cells. In this study, we demonstrate that SR-BI is a key host factor required for hepatitis C virus (HCV) uptake cross-presentation by human dendritic cells (DCs). Whereas monocytes T were characterized very low or undetectable expression levels, DCs demonstrated high level of cell surface similar primary hepatocytes. Antibodies targeting the extracellular loop efficiently inhibited HCV-like particle binding, uptake, DCs. Moreover, high-density lipoprotein specifically modulated binding DCs, indicating interplay HCV with transfer function Finally, anti-SR-BI antibodies inhibit culture-derived (HCVcc) conclusion, these findings identify novel viral antigen recognition may impact on design vaccines immunotherapeutic approaches aiming at induction efficient antiviral immune responses.