Herpes simplex virus virion host shutoff function

• DOI: 10.1128/jvi.62.3.912-921.1988
• Publication Date: 2020-01-06T14:51:39Z
• Authors: A D Kwong; J A Kruper; N Frenkel
• Keywords: Transcription

Herpes simplex virus (HSV) virions contain one or more functions which mediate the shutoff of host protein synthesis and degradation mRNA. HSV type 1 (HSV-1) mutants deficient in virion (vhs mutants) were isolated found to be defective their ability degrade Furthermore, it was that viral mRNAs cells infected with vhs mutant have a significantly longer functional half-life than wild-type virus-infected cells. In present study we mapped vhs1 mutation affecting 265-base-pair NruI-XmaIII fragment spanning map coordinates 0.604 0.606 HSV-1 genome. The mutation(s) half-lives mRNA as well alpha (immediate-early), beta (early), gamma (late) also within this fragment. Thus, is most likely mediated by same function decreases shorter infected-cell may allow rapid modulation gene expression response changes transcription genes. Interestingly, maps region overlaps Bg/II-N sequences HSV-2 DNA shown previously transform culture. possible relationship between transformation are discussed.