Determinants in the 5' noncoding region of poliovirus Sabin 1 RNA that influence the attenuation phenotype

• DOI: 10.1128/jvi.63.3.1302-1309.1989
• Publication Date: 2020-01-06T15:15:06Z
• Authors: N Kawamura; M Kohara; S Abe; T Komatsu; K Tago; M Arita; A Nomoto
• Keywords: Recombinant virus

A number of recombinants between the virulent Mahoney and attenuated Sabin strains type 1 poliovirus were constructed by using infectious cDNA clones two strains. To identify a strong neurovirulence determinant(s) residing in genome region upstream nucleotide position 1122, these recombinant viruses subjected to biological tests, including monkey tests. The results tests suggested important contribution an adenine residue (Mahoney type) at 480 expression phenotype poliovirus. This nucleotide, however, had only minor effect, if any, on viral temperature sensitivity. Monkey virus whose guanine (Sabin variants generated from this central nervous system monkeys strongly that one change, guanine, was not sufficient for full attenuation encoded region. These suggest depends highly ordered structure formed 5' noncoding sequence formation such is possibly influenced 480. Furthermore, vitro performed recovered injected with temperature-sensitive showing small-plaque d phenotypes revealed most even higher sensitivities all acquired large-plaque lost some extent. indicate there may be unknown selection pressure(s) common determinants might involved phenotypes.