A 10,400-molecular-weight membrane protein is coded by region E3 of adenovirus

• DOI: 10.1128/jvi.64.2.794-801.1990
• Publication Date: 2020-01-06T15:45:19Z
• Authors: A E Tollefson; P Krajcsi; S P Yei; C R Carlin; W S Wold
• Keywords: Immunoprecipitation

Previous studies with adenovirus mutants have indicated that a 10,400-molecular-weight (10.4K) protein predicted to be coded by an open reading frame in region E3 of functions down regulate the epidermal growth factor receptor (C. R. Carlin, A. E. Tollefson, H. Brady, B. L. Hoffman, and W. S. M. Wold, Cell 57:135-144, 1989). We now demonstrate 10.4K is fact synthesized cells infected group C adenoviruses. This was done immunoprecipitation from variety mutants, using antisera against three different synthetic peptides corresponding sequence. The translated primarily mRNA f, as cell-free translation purified hybridization RNA processing mutant synthesizes predominantly f. overproduced or underproduced vivo, respectively, overproduce underproduce also indicating migrated two bands apparent molecular weights 16,000 11,000 (10 18% gradient gels); both contained epitopes, shown Western blot (immunoblot). Only 16K band obtained translation, suggesting precursor 11K protein. membrane protein, cell fractionation experiments its sequence well putative N-terminal signal 30-residue transmembrane domain are conserved types 2 5 (group C) 3, 7, 35 B).