Ocular avirulence of a herpes simplex virus type 1 strain is associated with heightened sensitivity to alpha/beta interferon

• DOI: 10.1128/jvi.64.5.2187-2192.1990
• Publication Date: 2020-01-06T15:49:31Z
• Authors: Y H Su; J E Oakes; R N Lausch
• Keywords: Mice, Inbred BALB C; 0303 health sciences; Virulence; Immune Sera; In Vitro Techniques; Keratitis, Dendritic; Vesicular stomatitis Indiana virus; 3. Good health; Cornea; Kinetics; Mice; 03 medical and health sciences; Interferon Type I; Animals; Simplexvirus; Female

BALB/c mice infected on the scarified cornea with herpes simplex virus type 1 strain 35 [HSV-1(35)] rarely developed ocular disease even at challenge doses as high 10(7) PFU per eye. In contrast, HSV-1(RE) consistently induced stromal keratitis an inoculum of 2 x 10(4) PFU. The goal this study was to determine reason for difference in virulence between two HSV strains. Both HSV-1 strains replicated similar titers excised corneal "buttons." However, after vivo infection cornea, growth evident only during first 24 h postinfection, whereas replication RE persisted least 4 days. vitro tests revealed that HSV-1(35) greater than 10 times more sensitive alpha/beta interferon (IFN-alpha/beta) HSV-1(RE). comparable serum levels IFN intraperitoneal inoculation. kinetics clearance from eye markedly altered by treatment rabbit anti-IFN-alpha/beta. Virus exceeding could be demonstrated 5 days postinfection given a single inoculation antiserum infection. Furthermore, anti-IFN 3-week-old led development clinically apparent which subsequently progressed majority recipients. These results indicate striking capacity and induce related inherently sensitivity IFN-alpha/beta produced host response