Activation of transcription factor NF-kappaB by the Tat protein of human immunodeficiency virus type 1

• DOI: 10.1128/jvi.70.7.4427-4437.1996
• Publication Date: 2020-01-06T21:48:58Z
• Authors: F Demarchi; F d'Adda di Fagagna; A Falaschi; M Giacca
• Keywords: Transcription

A recombinant Tat protein was used to investigate the molecular mechanisms of transcriptional activation human immunodeficiency virus type 1 long terminal repeat (LTR). Liposome-mediated delivery this responsive cells results in dose-dependent LTR activation. As evaluated by mRNA quantitation with competitive PCR, response is rapid and transient, peaking at 5 h after beginning treatment. In vivo footprinting experiments showed that concomitant a modification protein-DNA interaction pattern downstream kappaB site enhancer adjacent Sp1 boxes. The effects on are mediated Tat-induced nuclear translocation NF-kappaB, which parallels kinetics This induction from degradation inhibitor IkappaB-alpha, blocked under antioxidant conditions protease inhibitor, occurs as different cell types. functional impaired upon treatment decoy or sodium salicylate, an NF-kappaB These show important for Furthermore, they suggest some pleiotropic cellular functions can be NF-kappaB.