Coxsackievirus and Adenovirus Receptor Amino-Terminal Immunoglobulin V-Related Domain Binds Adenovirus Type 2 and Fiber Knob from Adenovirus Type 12

• DOI: 10.1128/jvi.73.2.1392-1398.1999
• Publication Date: 2019-12-31T18:23:27Z
• Authors: Paul Freimuth; Karen Springer; Chris Berard; Jim Hainfeld; Maria Bewley; John Flanagan
• Keywords: Coxsackievirus; Mastadenovirus; Adenovirus infection

ABSTRACT The extracellular region of the coxsackievirus and adenovirus receptor (CAR) is predicted to consist two immunoglobulin (Ig)-related structural domains. We expressed isolated CAR amino-terminal domain (D1) a fragment containing both Ig domains (D1/D2) in Escherichia coli . Both D1 D1/D2 formed complexes vitro with recombinant knob type 12 (Ad12) fiber, inhibited 2 (Ad2) infection HeLa cells. These results indicate that adenovirus-binding activity localized IgV-related confirm our earlier observation Ad2 Ad12 bind same cellular receptor. Preliminary crystallization studies suggest bound will be suitable for structure determination.