Human Papillomavirus Type 16 E7 DNA Vaccine: Mutation in the Open Reading Frame of E7 Enhances Specific Cytotoxic T-Lymphocyte Induction and Antitumor Activity

• DOI: 10.1128/jvi.73.9.7877-7881.1999
• Publication Date: 2019-12-31T18:45:11Z
• Authors: Wei Shi; Ping Bu; Jianzhong Liu; Axel Polack; Susan Fisher; Liang Qiao
• Keywords: 0303 health sciences; Papillomavirus E7 Proteins; Papillomavirus Infections; Gene Expression; Viral Vaccines; Oncogene Proteins, Viral; Cancer Vaccines; Cell Line; 3. Good health; Mice, Inbred C57BL; Disease Models, Animal; Mice; Open Reading Frames; Tumor Virus Infections; 03 medical and health sciences; Mutagenesis; DNA, Viral; Vaccines, DNA; Animals; Humans; Papillomavirus Vaccines; Papillomaviridae; T-Lymphocytes, Cytotoxic

ABSTRACTA human papillomavirus type 16 E7 DNA vaccine with the open reading frame encoding mutations in two zinc-binding motifs expressed a rapidly degraded E7 protein. This vaccine induced a significantly stronger E7-specific cytotoxic T-lymphocyte response and better tumor protection in mice than did a wild-type E7 DNA vaccine expressing a stable E7 protein.