Classical Swine Fever Virus E rns Deletion Mutants: trans -Complementation and Potential Use as Nontransmissible, Modified, Live-Attenuated Marker Vaccines

• DOI: 10.1128/jvi.74.7.2973-2980.2000
• Publication Date: 2002-07-27T10:06:37Z
• Authors: M. N. Widjojoatmodjo; H. G. P. van Gennip; A. Bouma; P. A. van Rijn; R. J. M. Moormann
• Keywords: Pestivirus; Classical swine fever

An SK6 cell line (SK6c26) which constitutively expressed the glycoprotein E(rns) of classical swine fever virus (CSFV) was used to rescue CSFV deletion mutants based on infectious copy strain C. The biochemical properties from this were indistinguishable those E(rns). Two constructed, Flc23 and Flc22. Virus encoded only utmost N- C-terminal amino acids (deletion 215 acids) retain original protease cleavage sites. Flc22 is not recognized by a panel antibodies, due 66 in could be rescued vitro complementing SK6c26 cells. These viruses infect replicate cells but did yield virus. neutralization E(rns)-specific antibodies similar for wild-type recombinant viruses, indicating that incorporated viral particles. Pigs vaccinated with or protected against challenge lethal dose Brescia. This first demonstration trans-complementation defective pestivirus RNA pestiviral structural protein opens new ways develop nontransmissible modified live vaccines. In addition, absence (the antigenic part of) particles can differentiate between infected animals.