Virus Clearance through Apoptosis-Dependent Phagocytosis of Influenza A Virus-Infected Cells by Macrophages

• DOI: 10.1128/jvi.74.7.3399-3403.2000
• Publication Date: 2002-07-27T09:55:53Z
• Authors: Ippei Fujimoto; Jiehong Pan; Takenori Takizawa; Yoshinobu Nakanishi
• Keywords: 0301 basic medicine; Mice; Microscopy, Electron; 03 medical and health sciences; Phagocytosis; Influenza A virus; Macrophages; Animals; Humans; Apoptosis; HeLa Cells; 3. Good health

ABSTRACT Some cultured cell lines undergo typical apoptosis upon infection with influenza virus. However, the release of replicated virus into culture medium does not change when is inhibited. Since apoptotic cells are heterophagically eliminated at early stages pathway, we anticipated that coexistence phagocytic virus-infected affects extent growth. When A HeLa were mixed activated mouse peritoneal macrophages, efficient phagocytosis, which was abrogated in presence a caspase inhibitor, occurred. At same time, completely inhibited, and this required direct contact between macrophages. Furthermore, an immunoelectron microscopic analysis detected particles associated phagosome-like structures within These results indicate apoptosis-dependent phagocytosis may lead to elimination pathogen.