Fv-4: Identification of the Defect in Env and the Mechanism of Resistance to Ecotropic Murine Leukemia Virus

• DOI: 10.1128/jvi.75.22.11244-11248.2001
• Publication Date: 2002-07-27T09:55:53Z
• Authors: Gwen M. Taylor; Yi Gao; David Avram Sanders
• Keywords: Gammaretrovirus

ABSTRACT Mice expressing the Fv-4 gene are resistant to infection by ecotropic murine leukemia viruses (MuLVs). The encodes an envelope (Env) protein whose putative receptor-binding domain resembles that of MuLV Env protein. Resistance MuLVs appears result from viral interference involving binding endogenously expressed env -encoded receptor, although immune system also plays a role in resistance. is processed normally and can be incorporated into virus particles but unable promote entry. Among many sequence variations between transmembrane (TM) subunit TM subunits other proteins, there substitution arginine residue for glycine (gly-491 Moloney Env) otherwise conserved all MuLVs. This present fusion peptide sequence. In this study, gly-491 has been replaced with residues mutant bearing gly-487 was created. G491R recapitulates phenotype cell culture, indicating sufficient creation establish interference-mediated resistance produced gene. Analysis proteins implications understanding peptides engineering organismal retroviruses.