Infectivity and Replication Capacity of Drug-Resistant Human Immunodeficiency Virus Type 1 Variants Isolated during Primary Infection

• DOI: 10.1128/jvi.77.14.7736-7745.2003
• Publication Date: 2003-06-26T17:57:37Z
• Authors: Viviana Simon; Neal Padte; Deya Murray; Jeroen Vanderhoeven; Terri Wrin; Neil Parkin; Michele Di Mascio; Martin Markowitz
• Keywords: Infectivity; Resistance mutation

ABSTRACT It is believed that replication capacity an important determinant of human immunodeficiency virus type 1 (HIV-1) pathogenicity and transmissibility. To explore this, we conducted a comprehensive analysis the properties nine drug-resistant drug-susceptible viral isolates derived from patients with primary HIV-1 infection. Viral were tested for single-cycle infectivity in GHOST cell line. The carrying resistance-associated mutations was significantly higher than isolates. Additionally, growth kinetics these determined CD4 + T lymphocytes. Drug-resistant replicated as well viruses. Insertion resistance-conferring regions into NL4-3-based molecular background resulted chimeras displayed modest but significant reduction compared to chimeric Of note, two multidrug-resistant one protease inhibitor-resistant isolate rates other or These distinct replicative features, however, not seen corresponding chimeras, indicating changes within C-terminal region Gag reverse transcriptase genes contribute are sufficient level compensatory adaptation observed. findings suggest some viruses isolated during infection possess unique adaptive allow both high resistance more classes antiretroviral drugs. Further studies needed elucidate precise essential characteristics.