Comparing the Ex Vivo Fitness of CCR5-Tropic Human Immunodeficiency Virus Type 1 Isolates of Subtypes B and C

• DOI: 10.1128/jvi.77.2.1021-1038.2003
• Publication Date: 2002-12-26T19:42:51Z
• Authors: Sarah C. Ball; Awet Abraha; Kalonji R. Collins; Andre J. Marozsan; Heather Baird; Miguel E. Quiñon...; Adam Penn-Nicholson; Michael Murray; Nathalie Richard; Michael Lobritz; Peter A. Zimmerman; Tatsuyoshi Kawamura; Andrew Blauvelt; Eric J. Arts
• Keywords: Ex vivo

ABSTRACT Continual human immunodeficiency virus type 1 (HIV-1) evolution and expansion within the population have led to unequal distribution of HIV-1 group M subtypes. In particular, recent outgrowth subtype C in southern Africa, India, China has fueled speculation that isolates may be more fit vivo. this study, nine B six were added peripheral blood mononuclear cell cultures for a complete pairwise competition experiment. All less than ( P < 0.0001), but intrasubtype variations fitness not significant. Increased over was also observed primary CD4 + T cells macrophages from different donors skin-derived Langerhans cells. Detailed analysis retroviral life cycle during several competitions indicated efficiency host entry significant impact on relative fitness. Furthermore, phyletic analyses differences suggested that, recombined B/C isolate, higher mapped env gene rather gag pol genes. These results suggest transmitted with equal (Langerhans data) following initial infection (T-cell macrophage lead slower disease progression.