An External Loop Region of Domain III of Dengue Virus Type 2 Envelope Protein Is Involved in Serotype-Specific Binding to Mosquito but Not Mammalian Cells

• DOI: 10.1128/jvi.78.1.378-388.2004
• Publication Date: 2003-12-11T23:43:13Z
• Authors: Jan-Jong Hung; Meng-Ti Hsieh; Ming-Jer Young; Chuan-Liang Kao; Chwan-Chuen King; Wen Chang
• Keywords: Models, Molecular; 570; 0303 health sciences; Binding Sites; 610; Dengue Virus; Cell Line; 3. Good health; Structure-Activity Relationship; 03 medical and health sciences; Culicidae; Viral Envelope Proteins; Cricetinae; Animals; Receptors, Virus; Amino Acid Sequence; Serotyping

ABSTRACT Dengue virus (DV) is a flavivirus and infects mammalian cells through mosquito vectors. This study investigates the roles of domain III DV type 2 envelope protein (EIII) in binding to host cell. Recombinant EIII interferes with infection BHK21 C6/36 by blocking dengue virion adsorption these cells. Inhibition on was broad no serotype specificity; however, inhibition relatively specific. Soluble heparin completely blocks cells, suggesting that binds mainly cell surface heparan sulfates. suggestion supported observation very weakly gro2C sog9 mutant lines lack sulfate. In contrast, does not block Furthermore, synthetic peptide includes amino acids (aa) 380 389 EIII, IGVEPGQLKL, inhibits but corresponds lateral loop region E protein, indicating possible role this summary, results suggest plays an important addition, interacts sulfates when containing aa may participate