Analysis of Integration Sites of Jaagsiekte Sheep Retrovirus in Ovine Pulmonary Adenocarcinoma

• DOI: 10.1128/jvi.78.16.8506-8512.2004
• Publication Date: 2004-07-27T18:42:49Z
• Authors: Christina Cousens; Jeanette V. Bishop; Adrian W. Philbey; Clare A. Gill; Massimo Palmarini; Jonathan O. Carlson; James C. DeMartini; J. Michael Sharp
• Keywords: Insertional mutagenesis; Southern blot

ABSTRACT Ovine pulmonary adenocarcinoma (OPA) is an infectious lung tumor of sheep caused by Jaagsiekte retrovirus (JSRV). To test the hypothesis that JSRV insertional mutagenesis involved in oncogenesis OPA, we cloned and characterized 70 independent integration sites from 23 cases OPA. Multiple were identified most tumors. BLAST analysis sequences did not disclose any potential oncogenic motifs or identical different Thirty-seven mapped to individual chromosomes PCR with a panel sheep-hamster hybrid cell lines. Integration found on 20 28 chromosomes, suggesting random distribution. However, four tumors chromosome 16. By Southern blot hybridization, probes derived two these within 5 kb each other normal DNA. These single bacterial artificial clone further only 2.5 apart, uncharacterized predicted gene less than 200 mitogen-activated protein kinase-encoding gene. findings suggest there at least one common site for OPA add weight development this tumor.