The Amino-Terminal Region of Major Capsid Protein P3 Is Essential for Self-Assembly of Single-Shelled Core-Like Particles of Rice Dwarf Virus

• DOI: 10.1128/jvi.78.6.3145-3148.2004
• Publication Date: 2004-02-27T21:47:38Z
• Authors: Kyoji Hagiwara; Takahiko Higashi; Naoyuki Miyazaki; Hisashi Naitow; R. Holland Cheng; Atsushi Nakagawa; Hiroshi Mizuno; Tomitake Tsukihara; Toshihiro Omura
• Keywords: Models, Molecular; 0301 basic medicine; 0303 health sciences; Virus Assembly; Virion; Oryza; Reoviridae; Nucleopolyhedroviruses; Cell Line; 3. Good health; Structure-Activity Relationship; 03 medical and health sciences; Animals; Capsid Proteins; Dimerization; Gene Deletion

The core protein P3 of Rice dwarf virus constructs asymmetric dimers, one which is inserted by the amino-terminal region another protein. proteins with serial deletions, expressed in a baculovirus system, formed particles gradually decreasing stability. capacity for self-assembly disappeared when 52 amino acids had been deleted. These results demonstrated that insertion arm into appears to play an important role stabilizing particles.