Interaction of Moloney Murine Leukemia Virus Capsid with Ubc9 and PIASy Mediates SUMO-1 Addition Required Early in Infection

• DOI: 10.1128/jvi.80.1.342-352.2006
• Publication Date: 2005-12-13T02:52:45Z
• Authors: Andrew Yueh; Juliana Leung; Subarna Bhattacha...; Lucy A. Perrone; Kenia de los Santos; Szy-yuan Pu; Stephen P. Goff
• Keywords: Alanine scanning; Transcription; SUMO enzymes

Yeast two-hybrid screens led to the identification of Ubc9 and PIASy, E2 E3 small ubiquitin-like modifier (SUMO)-conjugating enzymes, as proteins interacting with capsid (CA) protein Moloney murine leukemia virus. The binding site in CA for was mapped by deletion alanine-scanning mutagenesis a consensus motif SUMOylation at residues 202 220, PIASy 114 176, directly centered on major homology region. Expression tagged SUMO-1 resulted covalent transfer vivo. Mutations lysine arginines near mutations reduced or eliminated SUMOylation. Introduction these into complete viral genome blocked virus replication. mutants exhibited no defects late stages gene expression virion assembly. Upon infection, mutant viruses were able carry out reverse transcription synthesize normal levels linear DNA but unable produce circular DNAs integrated provirus normally found nucleus. results suggest that mediated an interaction is required early events after before nuclear entry integration.