Novel Pol II Fusion Promoter Directs Human Immunodeficiency Virus Type 1-Inducible Coexpression of a Short Hairpin RNA and Protein

• DOI: 10.1128/jvi.80.4.1863-1873.2006
• Publication Date: 2006-01-26T18:02:43Z
• Authors: Hoshang J. Unwalla; Hai-Tang Li; Ingrid Bahner; Ming-Jie Li; Donald Kohn; John J. Rossi
• Keywords: Wild type

ABSTRACT We demonstrate a novel approach for coexpression of short hairpin RNA (shRNA) with an open reading frame which exploits transcriptional read-through minimal polyadenylation signal from Pol II promoter. first observed efficient inducible expression enhanced green fluorescent protein along anti-rev shRNA. took advantage this observation to test the transdominant negative mutant (humanized) human immunodeficiency type 1 (HIV-1) Rev (huRevM10) shRNA via HIV-1-inducible fusion The and resulted in potent, long-term inhibition HIV-1 gene suppression shRNA-resistant mutants. This dual system has broad-based potential other applications, such as cases where simultaneous knockdown wild-type transcripts must be accompanied by replacement protein.