The Two Functional Enoyl-Acyl Carrier Protein Reductases of Enterococcus faecalis Do Not Mediate Triclosan Resistance

• DOI: 10.1128/mbio.00613-13
• Publication Date: 2013-10-02T03:43:00Z
• Authors: Lei Zhu; Hongkai Bi; Jincheng Ma; Zhe Hu; Wenbin Zhang; John E. Cronan; Haihong Wang
• Keywords: Enterococcus faecalis; Acyl carrier protein; Fatty acid synthesis

Enoyl-acyl carrier protein (enoyl-ACP) reductase catalyzes the last step of elongation cycle in synthesis bacterial fatty acids. The Enterococcus faecalis genome contains two genes annotated as enoyl-ACP reductases, a FabI-type and FabK-type reductase. We report that expression either proteins restores growth an Escherichia coli fabI temperature-sensitive mutant strain under nonpermissive conditions. In vitro assays demonstrated both support acid are active with substrates all chain lengths. Although E. fabK confers to high levels resistance antimicrobial triclosan, deletion from showed FabK does not play detectable role inherent triclosan faecalis. Indeed, seems only minor modulating composition. Strains carrying grow normally without supplementation, whereas mutants make traces acids unsaturated auxotrophs.The finding exogenous strains defective indicates inhibitors ineffective countering infections because host serum bacterium.