Mapping the Regulatory Network for Salmonella enterica Serovar Typhimurium Invasion

• DOI: 10.1128/mbio.01024-16
• Publication Date: 2016-09-07T00:43:25Z
• Authors: Carol Smith; Anne M. Stringer; Chunhong Mao; Michael J. Palumbo; Joseph T. Wade
• Keywords: Salmonella enterica; Gene regulatory network; Pathogenicity island

ABSTRACT Salmonella enterica pathogenicity island 1 (SPI-1) encodes proteins required for invasion of gut epithelial cells. The timing is tightly controlled by a complex regulatory network. transcription factor (TF) HilD the master regulator this process and senses environmental signals associated with invasion. activates genes within outside SPI-1, including six other TFs. Thus, transcriptional program host cell at least 7 However, very few targets are known these TFs, extent network unclear. In study, we used complementary genomic approaches to map direct all Our data reveal highly interconnected that includes many previously undescribed targets. Moreover, extends well beyond due inclusion additional TFs noncoding RNAs. By comparing gene expression profiles identified uncharacterized likely play roles in We also uncovered cross talk between SPI-1 regulation pathways, which, turn, clusters share related functions. freely available through an intuitive online browser represent valuable resource bacterial research community. IMPORTANCE Invasion cells early step during infection requires secretion specific into via type III system (T3SS). Most T3SS-associated encoded horizontally acquired locus as (SPI-1). Multiple regulators respond ensure appropriate expression. particular, there seven be involved coordinating genes. have genome-scale regulators. target our functionally implicate pathways. All datasets browser.