Multicenter Outbreak of Infections by Saprochaete clavata , an Unrecognized Opportunistic Fungal Pathogen

• DOI: 10.1128/mbio.02309-14
• Publication Date: 2014-12-17T02:36:03Z
• Authors: Sophie Vaux; Alexis Criscuolo; Marie Desnos-Olli...; Laure Diancourt; Chloé Tarnaud; Matthias Vandenbo...; Sylvain Brisse; Bruno Coignard; Françoise Dromer; ; Dea Garcia-Hermoso; Catherine Blanc; Damien Hoinard; Olivier Lortholary; Stéphane Bretagne; Jean-Michel Thiolet; Henriette de Valk; Rémi Courbil; Anne Chabanel; Marion Simonet; Francoise Maire; Saadia Jbilou; Pierre Tiberghien; Hervé Blanchard; Anne-Gaëlle Venier; Claude Bernet; Loïc Simon; Hélène Sénéchal; Elodie Pouchol; Christiane Angot; Patricia Ribaud; G. Socié; M. Flèche; Nathalie Brieu; Evelyne Lagier; Vanessa Chartier; Thierry Allegre; Laurence Maulin; Hélène Lanic; Hervé Tilly; Jean-Philippe Bou...; Marc Pihet; Aline Schmidt; Achille Kouatchet; Yves-Marie Vandamme; Norbert Ifrah; Alain Mercat; Isabelle Accoceberry; Olivier Albert; Thibaut Leguay; Anne-Marie Rogues; Julie Bonhomme; Oumédaly Reman; Claire Lesteven; Philippe Poirier; Cécile Molucon Ch...; Laure Calvet; Olivier Baud; Monique Cambon; Jean Chistophe Fa...; Bruno Lafon; Frédéric Dalle; Denis Caillot; Aline Lazzarotti; Serge Aho; Sandrine Combret; Thierry Facon; Boualem Sendid; Séverine Loridant; Terriou Louis; Bruno Cazin; Bruno Grandbastien; Nathalie Bourgeois; Anne Lotthé; Guillaume Cartron; Christophe Ravel; Pascal Colson; Philippe Gaudard; Caroline Bonmati; Loic Simon; Christian Rabaud; Marie Machouart; Didier Poisson; Diana Carp; Jérôme Meunier; Anne Gaschet; Chantal Miquel; Laurence Sanhes; Milagros Ferreyra; Franck Leibinger; Philippe Geudet; Dominique Toubas; Chantal Himberlin; Florence Bureau-C...; Alain Delmer; Loïc Favennec; Gilles Gargala; Jean-Baptiste Michot; Christophe Girault; Marion David; Stéphane Leprêtre; Fabrice Jardin; Pierre Honderlick; Vincent Caille; Charles Cerf; Sophie Cassaing; Christian Recher; Muriel Picard; Caroline Protin; Françoise Huguet; Anne Huynh; Jean Ruiz; Béatrice Riu-Poulenc; Philippe Letocart; Bruno Marchou; Xavier Verdeil; Laurent Cavalié; Pamela Chauvin; Xavier Iriart; Alexis Valentin; Emmanuelle Bouvet; Béatrice Delmas-M...; Asma Jeblaoui; Najiby Kassis-Chi...; Konrad Mühlethaler; Stefan Zimmerli; Polona Zalar; Ferran Sánchez-Reus; Merce Gurgui
• Keywords: Isolation

ABSTRACT Rapidly fatal cases of invasive fungal infections due to a fungus later identified as Saprochaete clavata were reported in France May 2012. The objectives this study determine the clonal relatedness isolates and investigate possible sources contamination. A nationwide alert was launched collect cases. Molecular identification methods, whole-genome sequencing (WGS), clone-specific genotyping used analyze recent historical isolates, case-case performed. Isolates from thirty (26 fungemias, 22 associated deaths at day 30) collected between September 2011 October Eighteen occurred within 8 weeks (outbreak) 10 health care facilities, suggesting common source contamination, with potential secondary Phylogenetic analysis one clade (clade A), which accounted for 16/18 outbreak Results microbiological investigations environmental, drug, or food negative. Analysis exposures pointed medical device storage infusion blood products, but no contamination detected unused devices. previous studies demonstrated that S. can be found dairy products has already been involved monocentric outbreaks hematology wards. possibility may transmit through contaminated devices seen European is highly relevant management future newly recognized pathogen. This report also underlines further WGS investigation uncommon pathogens. IMPORTANCE Several rapidly short period time three 30 over 1 year, including an 18 weeks. Whole-genome (WGS) design clade-specific method uncovered clone outbreak, thus allowing risk factors infection by clone.