Modelling the Gastrointestinal Carriage of Klebsiella pneumoniae Infections

• DOI: 10.1128/mbio.03121-22
• Publication Date: 2023-01-04T14:00:50Z
• Authors: Ricardo Calderon-...; Alix Lee; Guillermo Lopez-C...; Steven J. Hancock; Joana Sa-Pessoa; Amy Dumigan; Ronan McMullan; Eric L. Campbell; Jose A. Bengoechea
• Keywords: Colonisation resistance

Klebsiella pneumoniae is a leading cause of nosocomial and community acquired infections, making K. the pathogen that associated with second largest number deaths attributed to any antibiotic resistant infection. colonizes nasopharynx gastrointestinal tract in an asymptomatic manner without dissemination other tissues. Importantly, colonization requisite for Our understanding still based on interrogating mouse models which animals are pretreated antibiotics disturb resistance imposed by gut microbiome. In these models, infections disseminate Here, we report murine model allow study tissue dissemination. Hypervirulent strains stably colonize inbred population treatment. The small intestine primary site followed transition colon over time, recapitulates disease dynamics metastatic able from sterile sites. Colonization mild moderate histopathology, no significant inflammation, effect richness sums up clinical scenario treatment disturbs results Finally, establish capsule polysaccharide necessary large intestine, whereas type VI secretion system contributes across tract. IMPORTANCE one pathogens sweeping world pandemic. healthy subjects manner, This makes it essential understand carriage preventing infections. Current research rely perturbation microbiome antibiotics, resulting invasive new key features human colonization. our model, there need microbiota achieve stable colonization, triggers We envision will be excellent platform upon investigate factors enhancing test therapeutics eliminate