The Demethylase JMJD2C Localizes to H3K4me3-Positive Transcription Start Sites and Is Dispensable for Embryonic Development

• DOI: 10.1128/mcb.00864-13
• Publication Date: 2014-01-07T02:26:20Z
• Authors: Marianne Terndrup...; Karl Agger; Anne Laugesen; Jens V. Johansen; Paul A. C. Cloos; Jesper Christensen; Kristian Helin
• Keywords: H3K4me3; Demethylase; Transcription

The histone demethylase JMJD2C, also known as KDM4C/GASC1, has activity against methylated H3K9 and H3K36 is amplified and/or overexpressed in human cancers. By the generation of Jmjd2c knockout mice, we demonstrate that loss compatible with cellular proliferation, embryonic stem cell (ESC) self-renewal, development. Moreover, report JMJD2C localizes to H3K4me3-positive transcription start sites both primary cells carcinoma KYSE150 line containing an amplification locus. Binding dependent on double Tudor domain which recognizes H3K4me3 but not H4K20me2/me3 vitro, showing a binding specificity different from domains JMJD2A JMJD2B. Depletion modest effect H3K9me3 H3K36me3 levels impairs proliferation leads deregulated expression subset target genes involved cycle progression. Taking these findings together, show targeted sites, where it can contribute transcriptional regulation, putative oncogene generally required for or