A Mouse Gene That Coordinates Epigenetic Controls and Transcriptional Interference To Achieve Tissue-Specific Expression

• DOI: 10.1128/mcb.01088-07
• Publication Date: 2007-11-13T02:06:06Z
• Authors: Alexandra C. Raca...; Fiona B. Turner; Lin-Ying Xie; Shirley M. Taylor; Richard G. Moran
• Keywords: CpG site; Transcription; RNA polymerase II

The mouse fpgs gene uses two distantly placed promoters to produce functionally distinct isozymes in a tissue-specific pattern. We queried how the P1 and P2 were differentially controlled. DNA methylation of CpG-sparse promoter occurred only tissues not initiating transcription at this site. promoter, which was embedded CpG island, appeared open all by several criteria, including lack methylation, yet used dividing tissues. patterns histone modifications over very different: P1, activation marks (acetylated histones H3 H4 trimethylated K4) reflected transcriptional activity apparently reinforced effects hypomethylated CpGs; P2, these present whether active, inactive, or engaged assembly futile initiation complexes. Since coexisted with silencing we sought mechanism interference. found RNA polymerase II, phosphorylated pattern consistent elongation, minimal levels factors liver. concluded that control expression from is dominant downstream masked occlusion.