lncRHOXF1, a Long Noncoding RNA from the X Chromosome That Suppresses Viral Response Genes during Development of the Early Human Placenta

• DOI: 10.1128/mcb.01098-15
• Publication Date: 2016-04-12T03:23:52Z
• Authors: Ian Penkala; Jianle Wang; Camille M. Syrett; Laura Goetzl; Carolina B. López; Montserrat C. Ang...
• Keywords: Sendai virus

Long noncoding RNAs (lncRNAs) can regulate gene expression in a cell-specific fashion during development. Here, we identify novel lncRNA from the X chromosome that named lncRHOXF1 and which is abundantly expressed trophectoderm primitive endoderm cells of human blastocyst-stage embryos. spliced polyadenylated about 1 kb length found both nuclear cytoplasmic compartments vitro differentiated progenitor cells. Gain-of-function experiments embryonic stem cells, normally lack RNA, revealed reduced proliferation favored cellular differentiation. knockdown using small interfering (siRNAs) progenitors increased viral response genes, including type I interferon. Sendai virus infection RNA levels, siRNA-mediated disruption genes leading to higher replication. Thus, first example regulates host infections placental propose it functions as repressor early