Deposition and Function of Histone H3 Variants in Tetrahymena thermophila

• DOI: 10.1128/mcb.01139-06
• Publication Date: 2006-10-03T00:16:29Z
• Authors: Bowen Cui; Yifan Liu; Martin A. Gorovsky
• Keywords: Transcription; Macronucleus

In Tetrahymena, HHT1 and HHT2 genes encode the same major histone H3; HHT3 HHT4 similar minor H3 variants (H3s), H3.3 H3.4. Green fluorescent protein (GFP)-tagged is deposited onto chromatin through a DNA replication-coupled (RC) pathway. GFP-tagged H3.4 can be both by transcription-associated, replication-independent (RI) pathway also weakly an RC Although types of H3s pathway, repair synthesis associated with meiotic recombination utilizes specifically. The regions distinguishing for their deposition pathways were identified. not essential. Cells grow without if are expressed at high levels. Surprisingly, cells lacking RI viable maintain normal nucleosome density highly transcribed region. detectably even when there no available, indicating that transcription-associated essential transcription. Minor required to produce sexual progeny play unexpected role in germ line micronuclei late conjugation unrelated