NADPH Oxidase 1 Controls the Persistence of Directed Cell Migration by a Rho-Dependent Switch of α2/α3 Integrins

• DOI: 10.1128/mcb.01199-08
• Publication Date: 2009-05-19T00:57:01Z
• Authors: Amine Sadok; Anne Pierres; Laetitia Dahan; Charles Prévôt; Maxime Lehmann; Hervé Kovacic
• Keywords: NOX1

NADPH oxidase 1 (Nox1) is expressed mainly in colon epithelial cells and produces superoxide ions as a primary function. We showed that Nox1 knockdown inhibits directional persistence of migration on collagen I. This paper dissects the mechanism by which affects direction colonic cell two-dimensional model. Transient activation during spreading temporarily inactivated RhoA led to efficient exportation alpha2beta1 integrin surface, supported persistent directed migration. loss takes place through RhoA-dependent alpha2/alpha3 switch. overactivation upon inhibition transient cytoskeletal reorganization increased cell-matrix contact associated with stable increase alpha3 surface expression. Blocking completely reversed In this model, would represent switch between random expression modulation.