Mds3 Regulates Morphogenesis in Candida albicans through the TOR Pathway

• DOI: 10.1128/mcb.01540-09
• Publication Date: 2010-05-11T04:10:13Z
• Authors: Lucia F. Zacchi; Jonatan Gomez-Raja; Dana A. Davis
• Keywords: Sirolimus; Biochemistry & Molecular Biology; 0303 health sciences; Base Sequence; Genes, Fungal; Gene Expression Regulation, Developmental; Cell Biology; Fungal Proteins; 03 medical and health sciences; Gene Expression Regulation, Fungal; Candida albicans; Mutation; Morphogenesis; Humans; DNA, Fungal; DNA Primers; Oligonucleotide Array Sequence Analysis; Signal Transduction

The success of Candida albicans as a major human fungal pathogen is dependent on its ability to colonize and survive commensal diverse mucosal surfaces. One trait required for survival virulence in the host morphogenetic yeast-to-hypha transition. Mds3 was identified regulator pH-dependent morphogenesis that functions parallel with classic Rim101 pH-sensing pathway. Microarray analyses revealed mds3 Delta/Delta cells had an expression profile indicative hyperactive TOR pathway, including preferential genes encoding ribosomal proteins decreased involved nitrogen source utilization. transcriptional morphological defects mutant were rescued by rapamycin, inhibitor TOR, this rescue lost strains carrying rapamycin-resistant TOR1-1 allele or rbp1 deletion. Rapamycin also associated loss Sit4, pathway effector, but not Ras1. sit4 mutants additional phenotypic similarities, suggesting Sit4 function similarly Finally, we found coimmunoprecipitate. Thus, new member contributes C. key