Fission Yeast Cid12 Has Dual Functions in Chromosome Segregation and Checkpoint Control

• DOI: 10.1128/mcb.02205-05
• Publication Date: 2006-06-01T00:31:17Z
• Authors: Thein Z. Win; Abigail L. Stevenson; Shao-Win Wang
• Keywords: 0301 basic medicine; Base Sequence; RNA Stability; Genes, Fungal; Molecular Sequence Data; Polynucleotide Adenylyltransferase; Cell Cycle Proteins; RNA, Fungal; DNA Polymerase II; Genes, cdc; Meiosis; 03 medical and health sciences; Chromosome Segregation; Mutation; Schizosaccharomyces; RNA Interference; Amino Acid Sequence; RNA, Messenger; Schizosaccharomyces pombe Proteins; DNA, Fungal; Gene Deletion; DNA Polymerase III

Fission yeast Cid12 is a member of the Cid1 family specialized poly(A) polymerases.Like cells lacking cid1, cid12⌬ mutants were shown to have checkpoint defects when DNA replication was inhibited.Here, we show that also required for faithful chromosome segregation and mutation amino acid residues predicted be essential polymerase activity resulted in loss function vivo.Cells had an increased failure rate due precocious sister chromatid cohesion at centromere but not along arms.In keeping with recently described RNA interference (RNAi)-mediated heterochromatin assembly, this accompanied by accumulation polyadenylated transcripts corresponding naturally silenced repeat elements within heterochromatic domains, consequent centromeric gene silencing.These suffered meiotic defects, their viability dependent on spindle protein Bub1.To account effects various aspects metabolism, including control, suggest has dual functions RNAi silencing regulating mRNA stability.