GTPase-Mediated Regulation of the Unfolded Protein Response in Caenorhabditis elegans Is Dependent on the AAA+ ATPase CDC-48

• DOI: 10.1128/mcb.02252-07
• Publication Date: 2008-05-06T00:24:46Z
• Authors: Marie-Elaine Caruso; Sarah Jenna; Marion Bouchecareilh; David L. Baillie; Daniel Boismenu; Dalia Halawani; Martin Latterich; Eric Chevet
• Keywords: Endomembrane system; Small GTPase

When endoplasmic reticulum (ER) homeostasis is perturbed, an adaptive mechanism triggered and named the unfolded protein response (UPR).Thus far, three known UPR signaling branches (IRE-1, PERK, ATF-6) mediate reestablishment of ER functions but can also lead to apoptosis if stress not alleviated.However, understanding molecular mechanisms integrating other functions, such as membrane traffic or endomembrane signaling, remains incomplete.We consequently sought identify new regulators UPR-dependent transcriptional focused on a family proteins mediate, among other, ER-related functions: small GTP-binding RAS superfamily.To this end, we used transgenic reporter Caenorhabditis elegans strains model specifically silence small-GTPase expression.We show that Rho subfamily member CRP-1 essential component UPR-induced events through its physical genetic interactions with AAA ؉ ATPase CDC-48.In addition, describe novel module involving CDC-48 which may directly link DNA remodeling transcription control.