Myc Regulates the Transcription of the PRC2 Gene To Control the Expression of Developmental Genes in Embryonic Stem Cells

• DOI: 10.1128/mcb.06148-11
• Publication Date: 2011-12-20T04:13:15Z
• Authors: Francesco Neri; Alessio Zippo; Anna Krepelova; Alessandro Cherubini; Marina Rocchigiani; Salvatore Oliviero
• Keywords: PRC2; Polycomb-group proteins; Ectopic expression; H3K4me3

Myc family members are critical to maintain embryonic stem cells (ESC) in the undifferentiated state. However, mechanism by which they perform this task has not yet been elucidated. Here we show that directly upregulates transcription of all core components Polycomb repressive complex 2 (PRC2) as well ESC-specific PRC2-associated factors. By expressing protein fused with estrogen receptor (Myc-ER) fibroblasts, observed Myc, binding regulatory elements Suz12, Ezh2, and Eed, induces acetylation histones H3 H4 recruitment elongating RNA polymerase II at their promoters. The silencing both c-Myc N-Myc ESC results reduced expression PRC2 H3K27me3 target developmental regulators upregulation genes involved primitive endoderm differentiation. ectopic ESC, either silenced for or induced differentiate leukemia inhibitory factor (LIF) withdrawal, is sufficient mark bivalent histone modifications keep repressed Thus, proteins control via complex.