Developmental Regulation of MicroRNA Expression in Schwann Cells

• DOI: 10.1128/mcb.06270-11
• Publication Date: 2011-11-08T09:00:12Z
• Authors: Nolan G. Gokey; Rajini Srinivasan; Camila Lopez-Anido; Courtney Krueger; John Svaren
• Keywords: SOX10; Dicer; Chromatin immunoprecipitation; Schwann cell; Remyelination

Schwann cell differentiation and subsequent myelination of the peripheral nervous system require action several transcription factors, including Sox10, which is vital at multiple stages development. The transition from immature to myelinating also regulated posttranscriptionally depends upon Dicer-mediated processing microRNAs (miRNAs). Although specific miRNA targets have begun be identified, mechanisms establishing dynamic regulation expression not been elucidated. We performed profiling studies identified 225 miRNAs differentially expressed during myelination. A subset 9 positively by miR-338 has implicated in oligodendrocyte maturation. In vivo chromatin immunoprecipitation (ChIP) sciatic nerve cells revealed a Sox10 binding site upstream an alternate promoter within Aatk gene, hosts miR-338. occupied this spinal cord ChIP experiments, suggesting similar regulatory mechanism oligodendrocytes. Cancer clusters that regulate proliferation, termed "oncomirs." cells, many these proproliferative was reduced absence Sox10. Finally, with oncomir increase CDK inhibitor p21 concomitant reduction proliferation.