Isolation of the gene encoding the Saccharomyces cerevisiae centromere-binding protein CP1.

• DOI: 10.1128/mcb.10.6.2458
• Publication Date: 2015-10-01T21:57:21Z
• Authors: R E Baker; D C Masison
• Keywords: Transcription

CP1 is a sequence-specific DNA-binding protein of the yeast Saccharomyces cerevisiae which recognizes highly conserved DNA element I (CDEI) centromeres. We cloned and sequenced gene encoding CP1. The codes for molecular weight 39,400. When expressed in Escherichia coli, directed synthesis CDEI-binding having same gel mobility as purified have given genetic designation CEP1 (centromere 1). was mapped found to reside on chromosome X, 2.0 centimorgans from SUP4. Strains were constructed most deleted. Such strains lacked detectable activity viable; however, disruption resulted 35% increase cell doubling time ninefold rate mitotic loss. An unexpected consequence methionine auxotrophy genetically linked cep1. This result recent finding that CDEI sites MET25 promoter are required activate transcription (D. Thomas, H. Cherest, Y. Surdin-Kerjan, J. Mol. Biol. 9:3292-3298, 1989) suggest both kinetochore factor.