Role of vacuolar acidification in protein sorting and zymogen activation: a genetic analysis of the yeast vacuolar proton-translocating ATPase.

• DOI: 10.1128/mcb.10.7.3737
• Publication Date: 2015-10-01T21:58:09Z
• Authors: C T Yamashiro; P M Kane; D F Wolczyk; R A Preston; T H Stevens
• Keywords: Intracellular pH; V-ATPase; Proton Transport

Vacuolar acidification has been proposed to play a key role in number of cellular processes, including protein sorting, zymogen activation, and maintenance intracellular pH. We investigated the significance vacuolar by cloning mutagenizing gene for yeast proton-translocating ATPase 60-kilodalton subunit (VAT2). Cells carrying vat2 null allele were viable; however, these cells severely defective growth medium buffered at neutral Vacuoles isolated from bearing completely devoid activity. The pH lumen mutation was 7.1, compared with wild-type 6.1, as determined flow cytometric assay. These results indicate that complex is essential low-pH state vacuole crucial normal growth. acidification-defective mutant exhibited activation but displayed minor defect sorting.