Expression of a 91-kilodalton PEA3-binding protein is down-regulated during differentiation of F9 embryonal carcinoma cells.

• DOI: 10.1128/mcb.12.5.2213
• Publication Date: 2015-10-06T00:30:00Z
• Authors: M E Martin; X Y Yang; W R Folk
• Keywords: Cell Nucleus; 0301 basic medicine; Base Sequence; Proto-Oncogene Proteins c-ets; Molecular Sequence Data; Cell Differentiation; Protein-Tyrosine Kinases; Cell Line; Proto-Oncogene Protein c-ets-2; DNA-Binding Proteins; Gene Expression Regulation, Neoplastic; Proto-Oncogene Protein c-ets-1; Repressor Proteins; Mice; 03 medical and health sciences; Enhancer Elements, Genetic; Oligodeoxyribonucleotides; Proto-Oncogene Proteins; Animals; Drosophila; RNA, Messenger; RNA, Neoplasm

Proteins binding to the PEA3 enhancer motif (AGGAAG) activate polyomavirus early promoter and help comprise viral late mRNA initiator element (W. Yoo, M. E. Martin, W. R. Folk, J. Virol. 65:5391-5400, 1991). Because many developmentally regulated cellular genes have motifs near their sequences, because Ets family gene products motif, we studied expression of PEA3-binding proteins Ets-related during differentiation F9 embryonal carcinoma cells. An approximately 91-kDa protein (PEA3-91) was identified in cell nuclear extracts by UV cross-linking a radiolabeled oligonucleotide probe, PEA3-91 down-regulated after cells parietal endoderm. The c-ets-1 product binds sequence murine sarcoma virus long terminal repeat that is similar (cGGAAG), but not cross-linked this Ets-1-binding nor did antiserum which recognizes c-ets-2 any effect on activity mobility shift assays. Furthermore, detected undifferentiated or differentiated cells, levels remained high differentiation. Antiserum against Drosophila E74A protein, however, recognized an 92-kDa whose varied manner identical PEA3-91. These data suggest ets-1 ets-2 likely be homolog E74 gene.