The promoter region of the yeast KAR2 (BiP) gene contains a regulatory domain that responds to the presence of unfolded proteins in the endoplasmic reticulum.

• DOI: 10.1128/mcb.13.2.877
• Publication Date: 2015-10-06T00:33:16Z
• Authors: K Kohno; K Normington; J Sambrook; M J Gething; K Mori
• Keywords: 0301 basic medicine; Hot Temperature; Transcription, Genetic; Protein Conformation; Genes, Fungal; Molecular Sequence Data; Down-Regulation; Saccharomyces cerevisiae; Regulatory Sequences, Nucleic Acid; Endoplasmic Reticulum; Fungal Proteins; 03 medical and health sciences; Gene Expression Regulation, Fungal; HSP70 Heat-Shock Proteins; RNA, Messenger; Cloning, Molecular; DNA, Fungal; Promoter Regions, Genetic; Heat-Shock Proteins; Sequence Deletion; 0303 health sciences; Base Sequence; Blotting, Northern; 3. Good health

The endoplasmic reticulum (ER) of eukaryotic cells contains an abundant 78,000-Da protein (BiP) that is involved in the translocation, folding, and assembly secretory transmembrane proteins. In yeast Saccharomyces cerevisiae, as mammalian cells, BiP mRNA synthesized at a high basal rate further induced by presence increased amounts unfolded proteins ER. However, unlike BiP, also severalfold heat shock, albeit transient fashion. To identify regulatory sequences respond to these stimuli KAR2 gene encodes we have cloned 1.3-kb segment DNA from region upstream coding for fused it reporter gene, Escherichia coli beta-galactosidase gene. Analysis series progressive 5' truncations well internal deletions sequence showed information required accurate transcriptional regulation S. cerevisiae contained within approximately 230-bp XhoI-DraI fragment (nucleotides -245 -9) this least two cis-acting elements, one (heat shock element [HSE]) responding other (unfolded response [UPR]) HSE UPR elements are functionally independent each but work additively maximum induction Lying between GC-rich similar consensus binding transcription factor Sp1 expression Finally, provide evidence suggesting monitor concentration free ER adjust level accordingly; effect mediated via promoter.