Identification of a novel interleukin-6 response element containing an Ets-binding site and a CRE-like site in the junB promoter.

• DOI: 10.1128/mcb.13.5.3027
• Publication Date: 2015-10-06T00:34:27Z
• Authors: K Nakajima; T Kusafuka; T Takeda; Y Fujitani; K Nakae; T Hirano
• Keywords: JUNB; Response element; AP-1 transcription factor

Interleukin-6 (IL-6) activation of the immediate-early gene junB has been shown to require both a tyrosine kinase and an unknown 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H7)-sensitive pathway. Here we report identification characterization IL-6 response element in promoter (designated JRE-IL6) HepG2 cells. The JRE-IL6 element, located at -149 -124, contains two DNA motifs, Ets-binding site (EBS) (CAGGAAGC) CRE-like (TGACGCGA). Functional studies using variously mutated elements showed that motifs were necessary sufficient for promoter. EBS (JEBS) appears bind protein Ets family or related which could also form major complex with EBSs murine sarcoma virus long terminal repeat human T-cell leukemia type 1 repeat. weakly multiple CREB-ATF proteins. Despite similarity structure between polyomavirus enhancer PyPEA3, composed AP1-binding known be activated by variety oncogene signals, not Ha-Ras, Raf-1, 12-O-tetradecanoylphorbol-13-acetate. We show activates through H7-sensitive pathway does involve C, cyclic AMP-dependent kinase, Ca(2+)- calmodulin-dependent kinases, Ras, NF-IL6 (C/EBP beta). combination JEBS basis selective efficient but signals generated C.