An E-box element localized in the first intron mediates regulation of the prothymosin alpha gene by c-myc.

• DOI: 10.1128/mcb.14.6.3853
• Publication Date: 2015-10-06T00:40:01Z
• Authors: S Gaubatz; A Meichle; M Eilers
• Keywords: 0301 basic medicine; Genomic Library; Base Sequence; Molecular Sequence Data; Genes, myc; DNA; Fibroblasts; Polymerase Chain Reaction; Proto-Oncogene Mas; Introns; Blotting, Southern; Kinetics; 03 medical and health sciences; Gene Expression Regulation; Cricetinae; Consensus Sequence; Animals; Humans; Cloning, Molecular; Protein Precursors; Luciferases; DNA Primers

In RAT1A fibroblasts, expression of the prothymosin alpha gene is under transcriptional control c-myc proto-oncogene. We have now cloned rat encoding and show that regulated by in vivo. find regulation mediated sequences downstream start site, whereas promoter constitutive not c-myc. identified an enhancer element within first intron sufficient to mediate a response Myc Max transient transfection assays activation estrogen receptor-Myc chimeras this contains consensus Myc-binding site (CACGTG). Disruption abolishes both stable assays. Mutants either or are deficient for heterodimerization fail regulate gene, suggesting heterodimer between activates gene. Our data define as bona fide target