Characterization of constitutive HSF2 DNA-binding activity in mouse embryonal carcinoma cells.

• DOI: 10.1128/mcb.14.8.5309
• Publication Date: 2015-10-06T00:40:34Z
• Authors: S P Murphy; J J Gorzowski; K D Sarge; B Phillips
• Keywords: Heat shock factor; HSF1; Transcription

Two distinct murine heat shock transcription factors, HSF1 and HSF2, have been identified. mediates the transcriptional activation of genes in response to environmental stress, while function HSF2 is not understood. Both factors can bind elements (HSEs) but are maintained a non-DNA-binding state under normal growth conditions. Mouse embryonal carcinoma (EC) cells only mammalian known exhibit HSE-binding activity, as determined by gel shift assays, even when at physiological temperatures. We demonstrate here that constitutive activity present F9 PCC4.aza.R1 EC cells, well similar found be mouse embryonic stem composed predominantly HSF2. trimerized higher levels than variety nonembryonal cell lines, suggesting correlation these properties with activity. Surprisingly, run-on assays suggest unstressed does stimulate two putative target genes, hsp70 hsp86. Genomic footprinting analysis indicates bound vivo HSE promoter although nucleus accessible other following shock. Thus trimerization nuclear localization do appear sufficient for binding cells.