A Minimal Spliceosomal Complex A Recognizes the Branch Site and Polypyrimidine Tract

• DOI: 10.1128/mcb.17.5.2944
• Publication Date: 2015-10-09T22:07:41Z
• Authors: Charles C. Query; Patrick S. McCaw; Phillip A. Sharp
• Keywords: snRNP; Polypyrimidine tract; Spliceosome; Polypyrimidine tract-binding protein; Small nuclear RNA

The association of U2 snRNP with the pre-mRNA branch region is a critical step in assembly spliceosomal complexes. We describe an process that reveals both minimal requirements for formation snRNP-substrate RNA complex, here designated Amin and specific interactions site adenosine. substrate oligonucleotide, containing only sequence polypyrimidine tract. Interactions at adenosine tract-binding proteins complex are same as those authentic prespliceosome A. Surprisingly, does not require U1 or ATP, suggesting these factors necessary stable binding per se, but rather accessibility components on longer substrates. Furthermore, there ATP-dependent activity releases destabilizes from sequences. simplicity will facilitate detailed understanding prespliceosomes.